Identifying Micro-Organisms

AIM: to practice using some sterile techniques, to derive a range of micro-organisms found in note macroscopically and to examine a range of micro- organisms found in victuals and water supply macroscopically APPARATUS:         Incubator          aseptic nutritive agar plates 1.         3 water ensamples 2.         1 air sample 3.         3 food samples          etna burner         Sterile pipettes          asseverate loops         Sticky tape         Marking tape         Water samples(various)          yoghurt         Inoculating needle/ probe          sick from fruit, vegetables or loot         Methylated strong drink RISK ASSESMENT: 1.         Glasswargon - throne cause cuts if handled incorrectly 2.         Bunsen burner - can cause destroy if not on caoutchouc blaze 3.         Methylated strong drink - toxic if ingested, highly combustible. Skin irritant 4.         Although the micro-organisms utilize in the activities in this manual are considered safe, standard microbiological appendage requires that they are regarded as potentially harmful SAFETY PROCEDURES: 1.         Handle with care, do not founder alone glassware near the bound of the bench 2.         take care, do not leave flame unattended keep flammable liquid i.e. Methylated hard liquor away 3.         Keep Methylated spirits away from flames. suffer gloves when handling it 4.         Wear gloves, lab coat and safety glasses. every used equipment should be placed in disinfectant.

Dispose of cultures by and by use, in an autoclave. Wash hands forrader leaving lab. neer open agar plate after setting up METHOD: 1.         Sterilise the workbench area by take up alcohol on to it 2.         collect 8 sterile alimental agar plates 3.         A sample of uncontaminating milk was obtained 4.         leave one plate aside, certain and taped as the stop 5.          use a sterile inoculating loop, a micro sample of the fresh milk was streaked onto the plates. This plate was thus sealed with sticky tape and labeled as fresh milk 6.          travel 3 and 4 were then tell with tap water, distilled water, seek tank water, fresh milk, old milk, If you essential to get a full essay, order it on our website: BestEssayCheap.com

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